Engineered Heart Tissue

The generation of miniature Engineered Heart Tissue (EHT) (Hansen, Eder et al. 2010) involves construction using fibrinogen and thrombin based hydrogels in 24-well dishes, and two silicone posts per well to allow muscle tissue to attach in culture. Spontaneous or electrically stimulated contractions of the tissue deflect the posts and are studied by video-optical recording.

Contraction analysis of EHTs is carried out in a gas and temperature controlled incubator with a glass roof and a video camera mounted onto a motorized xyz-axis system. A proprietary software controls the positioning of the camera above each well of the culture dish and records the movement of the EHT over a set time. This system is being used in our lab to model the human heart as we study the phenotype effects resulting from gene programme modulation.

Engineered Heart Tissue (EHT). EHTs are three dimensional force-generating cardiac tissue structures. Engineered heart tissues, in a strip format, generate force under auxotonic stretch conditions. 10–15 d after casting, EHTs can be used for contractility measurements

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