CRISPR/CAS9 Genome Editing
The Foo lab is using CRISPR/Cas9 tools in different ways, both in vitro & in vivo. We do this to deploy gene knockout & deletions, transgene & reporter insertions via HDR, generation of point mutations via base-editing & HDR, CRISPR-activation & inhibition of genes, as well as live imaging of chromosomal loci with fluorescently tagged dCas9.
We have harnessed techniques & tools published by other leading research groups and have adapted and developed these tools in our own hands to obtain efficient targeting in e.g. human ES cells, as well as in the in vivo mouse heart.